Statistics Seminar: Alicia Oshlack -- Dealing with the data deluge: differential expression analysis for RNA-seq

Alicia Oshlack
Murdoch Childrens Research Institute
The Royal Children’s Hospital

Location: Carslaw 173

Time:  2pm Friday, September 23, 2011

Dealing with the data deluge: differential expression analysis for RNA-seq

Abstract: 
New high-throughput DNA sequencing technologies have been rapidly developing
over the past 5 years and are routinely able to produce millions of short
DNA reads. These technologies are being widely used to study the
transcriptome in which steady state RNA is sequenced (RNA-seq). These
technologies produce vast amounts of data which need to be analysed in order
to make biological inference. RNA-seq data are complex and the analysis
involves a series of steps for which research is ongoing. In this talk I
will describe the data and outline the major steps involved in analysing an
RNA-seq dataset, focusing on methods to determine differentially expressed
genes between samples and to perform gene set testing.